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Structures, Functions, and Interactions of SARS-CoV related proteins

 Legend

 Title  Structure Image  Structure Detail

 Function

 Interactions

 Biological Role

 nsp1

nsp1 New Fold : PDBID: 2GDT
Not Known
In MHV, nsp1 interacts and/or co-localizes with counterparts of nsp2, 5, 7, 8, 9, 10, 12, 13 and sars9a.
Not Known

 nsp2

nsp2 N/A
test
In MHV, it interacts and/or co-localizes with counterparts of nsp1, 5, 7, 8, 9, 10, 12, 13, and sars9a
Not Known

 nsp3aUB1

nsp3aUB1 2GRI
Not Known
Not Known
Not Known

 nsp3bADRP

nsp3bADRP Macro-H2A-like fold
ADP-Ribose-1'-Phosphate phosphatase
ADP-Ribose-1'-Phosphate
Terminal step in splicing pathway during synthesis of nested set of sub-genomic mRNAs

 nsp3cSUD

nsp3cSUD N/A
Putative protease
Not Known
Not Known

 nsp3dPLP2

nsp3dPLP2 PDB ID: 2FE8 Cysteine protease and ubiquitin-like domains.
Cysteine protease activity.
pp1a
Proteolytic processing of pp1a/b. Potential de-ubiquitination of SARS proteins.

 nsp3eRNABD

nsp3eRNABD N/A
Not Known
Not Known
Not Known

 nsp3fZn-finger

nsp3fZn-finger Quadruple pass TM domain
Not known
Not known
Not known

 nsp3gY

nsp3gY N/A
Not Known
Not Known
Not Known

 nsp4

nsp4 Helical Transmembrane Protein
Not determined.
Not known
Not known

 nsp5

nsp5 Chymotrypsin-like protease fold.
Main SARS protease. Cleaves the replicase polyprotein to generate mature non-structural proteins nsp4-nsp16
Not known
Proteolytic processing of replicase polyprotein.

 nsp6

nsp6 Likely to have seven integral transmembrane helices
Not known
Not known
Not known

 nsp7

nsp7 Helical bundle
Forms a supercomplex of sixteen subunits with eight copies of itself and eight copies of nsp8
Interacts with nsp8 and possibly with RNA polymerase
Not clear

 nsp8

nsp8 New fold.
Eight copies of interacts with eight copies of nsp7 to form a supercomplex. Possible role of this supercomplex might be to channel RNA through to the polymerase for replication/transcription.
interacts with RNA as well as with nsp7.
RNA guide during Replication and transcription.

 nsp9

nsp9 Novel fold related to second domain of SARS-3CL-pro
RNA binding protein
Not characterized.
Unknown but possibly involves some form of RNA binding phenomenon.

 nsp10

nsp10 Novel fold with two zinc fingers.
RNA binding protein that forms a dodecamer.
Interacts with RNA in a sequence non-specific manner.
Two possible biological roles have been attributed. (a) It is probably a scaffold for RNA during replication by the SARS polymerase and (b) It is a novel viral transcription factor.

 nsp11

nsp11 11 residue peptide. Most likely unstructured.
Not known but likely associated with RNA polymerase
Possibly interacts with nsp10 and/or nsp12 (The RNA dependent RNA polymerase.
Unknown.

 nsp12

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 nsp13

nsp13 Not Known.
helicase activity. (Unwinds double stranded regions of genomic and subgenomic RNA during replication.
Not characterized.
helicase activity during genome replication.

 nsp14

nsp14 N/A
Exhibits 3'->5' exonuclease activity. Probably provides proof reading capability to the Replicase machinery.
None characterized yet.
Exonuclease activity during replication.

 nsp15

nsp15 Novel Fold: NendoU/XendoU
Uridylate specific endonuclease
Not characterized.
Processing and maturation of genomic and subgenomic RNA

 nsp16

nsp16 N/A
Putative methyltransferase
Not identified yet.
2'-O ribose cap methylation of viral genomic RNA

 sars2

sars2 Helical fusion core, HR1 and HR2 repeats are also helical.
Recognition of ACE2 receptor and viral entry into infected cells. Major glycoprotein of the virion coat.
Interacts with ACE2 receptor on human respiratory cells. Interacts with Matrix and N proteins. Possibly also with small envelope.
Viral entry during pathogenesis.

 sars3a

nsp3a Not Known
Unclear
Unclear
Not Known

 sars3b

sars3b Not Known
Not Known
Not Known
Not Known

 sars4

sars4 Integral transmembrane protein
Structural protein of the virion. Induces apoptosis in infected cells.
Possibly interacts with spike and/or matrix structural proteins.
Viral Structural glycoprotein.

 sars5

sars5 Structure not determined yet.
Adundant viral structural glycoprotein.
Interacts with Spike as well as the N (Nucleocapsid) protein.
Protein scaffold within the pleomorphic lipidic virion coat.

 sars6

sars6 Structure Not known.
Not known
Not known
Not known

 sars7a

sars7a beta sandwich fold.
Type I integral membrane protein
Not determined.
Not Known

 sars7b

sars7b Not known
Not known
Not known
Not known

 sars8

sars8 Not known
Not known
Not known
Not known

 sars9a

sars9a New fold observed at both the N terminal RNA binding domain as well as the C-terminal oligomerization domains.
Encases the genomic RNA and packages it into mature virions.
Interacts with both genomic as well as sub-genomic RNAs. Further, it interacts with the C-terminus of Matrix (M) protein underneath the viral coat.
Genomic RNA packaging and forming higher order structures.

 sars9b

sars9b Not known
Not known
Not known
Not known

 sars14

sars14 Not known
Not known
Not known
Not known