SARS-CoV

Funding for FSPS is provided through NIH-NIAID Contract HHSN266200400058C, Biodefense Proteomics Research Programs: Identifying Targets for Therapeutic Interventions Using Proteomic Technology.

The goal of FSPS is to provide a comprehensive molecular characterization and cataloging of all SARS-CoV related proteins and associated functions at the viral and host levels. FSPS will provide the required knowledge, experimental protocols and materials to enable the development of therapeutic interventions by achieving the following objectives:

• Bioinformatics discovery and comprehensive proteome analysis for the functional mapping of the SARS-CoV proteome
• Create and maintain a SARS-CoV expression-ready clone library containing all known and predicted coding regions for all available strains. The expression-ready clone library will be constructed using experimentally validated clones for E. coli, baculovirus and mammalian expression systems.
• Determine the function and biological role of SARS-CoV and related proteins. This includes the characterization of the life cycle and host response, the identification and characterization of protein-protein interactions, and the identification and characterization of ligands
• Comprehensive three-dimensional structure determination of these proteins by NMR and X-ray crystallography using rapid optimization through variations in clones, expression systems, and crystallization conditions
• Bioinformatics prediction and correlation will be used to integrate the functional and structural data generated by the FSPS project and other groups